Margot Robbie: Harley Quinn

Rosie Perez: Renee Montoya

Mary Elizabeth Winstead: Helena Bertinelli, The Huntress

Jurnee Smollett: Dinah Lance, Black Canary

Ewan McGregor: Roman Sionis

Ella Jay Basco: Cassandra Cain

Chris Messina: Victor Zsasz

Ali Wong: Ellen Yee

David Ury: Sleazy Breeder

Sara Montez: Shallow Friend #1, Kathrine

Izabel Pakzad: Shallow Friend #2

Daniel Bernhardt: Sionis' Chauffeur

Kc Strubbe: Naïve Teammate

Jacky Shu: Skeptical Teammate

Paloma Rabinov: Bitchy Teammate

Derek Wilson: Detective Tim Monroe