Tom Holland: Peter Parker, Spider-Man

Michael Keaton: Adrian Toomes, Vulture

Robert Downey Jr.: Tony Stark, Iron Man

Marisa Tomei: May Parker

Jon Favreau: Happy Hogan

Gwyneth Paltrow: Pepper Potts

Zendaya: Michelle

Donald Glover: Aaron Davis

Jacob Batalon: Ned

Laura Harrier: Liz

Tony Revolori: Flash

Bokeem Woodbine: Herman Schultz, Shocker #2

Tyne Daly: Anne Marie Hoag

Abraham Attah: Abe

Selenis Leyva: Ms. Warren

Angourie Rice: Betty

Martin Starr: Mr. Harrington

Michael Chernus: Phineas Mason, The Tinkerer

Michael Mando: Mac Gargan

Logan Marshall-Green: Jackson Brice, Shocker #1