Spider-Man/Peter Parker : Tobey Maguire.

Mary Jane Watson : Kirsten Dunst

Norman Osborn/The Green Goblin : Willem Dafoe

Harry Osborn : James Franco

Aunt May : Rosemary Harris

Uncle Ben : Cliff Robertson

J. Jonah Jameson : J.K. Simmons

Flash Thompson : Joe Manganiello

Robbie Robertson : Bill Nunn